Purified from Pichia pastoris culture by mAb affinity chromatography. Purity >95% by SDS-PAGE.
See product insert.
Preservative-free and carrier-free in phosphate buffered saline, pH 7.4. Filtered through 0.22µm filter.
Best EA, Stedman KE, Bozic CM, Hunter SW, Vailes LD, Chapman MD, McCall CA, McDermott MJ. A recombinant group 1 house dust mite allergen. rDer f 1, with biological activities similar to those of the native allergen. Prot Exp Pur 2000; 20:462-471.
Chapman MD, Smith AM, Vailes LD, Arruda LK, Dhanaraj V, Pomés A. Recombinant allergens for diagnosis and therapy of allergic disease. J Allergy Clin Immunol 2000; 106:409-418.
Chruszcz M, Chapman MD, Vailes LD, Stura EA, Saint-Remy JM, Minor W, Pomés A. Crystal structures of mite allergens Der f 1 and Der p 1 reveal differences in surface-exposed residues that may influence antibody binding. J Mol Biol, 2009;386:520-530.