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    产品货号:EL-DP1
    促销: 12720.00
    价格: 12720.00

    分享

    kit
    美国Indoor

    ELISA kit for measuring D.   pteronyssinus allergen, Der p 1.

    Antibodies:

    5H8/4C1

    Sensitivity:

    0.5ng/ml

    Limit of Detection:

    2ng/ml

    Assay Performance:

    Intra-assay CV% of 6.4; Inter-assay CV% of 15

    Contents:

    Monoclonal antibody 5H8, Universal   Allergen Standard, Biotinylated antibody 4C1; sufficient reagents for 10   plates.

    Storage:

    Store at 4°C.

    Not Provided:

    50mM Carbonate/Bicarbonate buffer, pH 9.6

      Phosphate Buffered Saline, pH 7.4, containing 0.05% Tween 20 (PBS-T)

      Bovine Serum Albumin

      Streptavidin-Peroxidase

      1mM ABTS in 70mM citrate-phosphate buffer, pH 4.2

    Notes:


    For research and commercial use in vitro: not for   human in vivo or therapeutic use.


    Certificate of Analysis

    Monoclonal Antibody:

    5H8 (clone 5H8 C12 D8)

    Vial:

    Red Cap; 100µl

    Immunogen:

    Der p 1

    Isotype:

    Mouse IgG2A

    Specificity:

    Binds to an epitope present on Dermatophagoides   pteronyssinusallergen, Der p 1.

    Purification:

    Produced in tissue culture and purified by affinity   chromatography using Protein A. Single heavy and light chain bands on   SDS-PAGE.

    Concentration:

    2.0mg/ml in phosphate buffered saline, pH 7.4.    Based on A280 for IgG (1.42=1mg/ml), 0.22µm filtered, preservative   free.

     
     

    Monoclonal Antibody:

    4C1 (clone 4C1 B8 3F8)

    Vial:

    Brown Cap; 100µl

    Immunogen:

    Der f 1

    Isotype:

    Mouse IgG1

    Specificity:

    Binds to an epitope on dust mite Dermatophagoides Group   1 allergens (Der f 1, Der p 1, Der m 1, Eur m 1).

    Purification:

    Produced in ascites and purified by affinity   chromatography using Protein A. Single heavy and light chain bands on   SDS-PAGE.

    Biotinylation:

    Biotinylated and titrated for use in ELISA at 1/1000   dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22µm filtered,   preservative free.

     
     

    Allergen Standard:

    Universal Allergen Standard

    Vial:

    White Cap; 400µl

    Composition:

    A formulation of eight purified natural allergens   prepared in 1% BSA/50% glycerol/PBS, pH 7.4.

    Concentration/Calibration:


    Universal Allergen Standard

    Protein Measurement

    Concentration (ng/ml)

    Der p 1

    Amino-acid analysis

    2500

    Der f 1

    Amino-acid analysis

    2500

    Der p 2

    Amino-acid analysis

    1000

    Fel d 1

    Amino-acid analysis

    1000

    Can f 1

    Amino-acid analysis

    2500

    Rat n 1

    Amino-acid analysis

    1000

    Mus m 1

    Amino-acid analysis

    250

    Bla g 2

    Amino-acid analysis

    2500

    References:



    1. Luczynska CM, Arruda LK, Platts-Mills TA,   Miller JD, Lopez M, Chapman MD. A two-site monoclonal antibody ELISA for the   quantification of the major Dermatophagoides spp. allergens, Der p I and Der   f I. J Immunol Methods 1989; 118(2):227-235.

    2. Custovic A, Taggart SC, Francis HC, Chapman MD,   Woodcock A. Exposure to house dust mite allergens and the clinical activity   of asthma. J Allergy Clin Immunol 1996; 98(1):64-72.

    3. Platts-Mills TA, Vervloet D, Thomas WR, Aalberse RC,   Chapman MD. Indoor allergens and asthma: report of the Third International   Workshop. J Allergy Clin Immunol 1997; 100(6 Pt 1):S2-24.

    4. Sawyer G, Kemp T, Shaw R et al. Biologic pollution   in infant bedding in New Zealand: high allergen exposure during a vulnerable   period. J Allergy Clin Immunol 1998; 102(5):765-770.

    5. Arbes SJ, Jr., Cohn RD, Yin M et al. House dust   mite allergen in US beds: results from the First National Survey of Lead and   Allergens in Housing. J Allergy Clin Immunol 2003; 111(2):408-414.

    6. Gruchalla RS, Pongracic J, Plaut M et al. Inner City   Asthma Study: relationships among sensitivity, allergen exposure, and asthma   morbidity. J Allergy Clin Immunol 2005; 115(3):478-485.

    7. Zock JP, Heinrich J, Jarvis D et al. Distribution   and determinants of house dust mite allergens in Europe: the European   Community Respiratory Health Survey II. J Allergy Clin Immunol 2006;   118(3):682-690.

    8. Jarvis D, Zock JP, Heinrich J et al. Cat and dust   mite allergen levels, specific IgG and IgG(4), and respiratory symptoms in   adults. J Allergy Clin Immunol 2007; 119:697-704.

    9. Earle CD, King EM, Tsay A et al. High-throughput   fluorescent multiplex array for indoor allergen exposure assessment. J   Allergy Clin Immunol 2007; 119(2):428-433.

    (For complete reference list see EL-DP1 Certificate of Analysis)