Monoclonal antibody 4B10, Bet v 1 Standard, Biotinylated antibody 2E10; sufficient reagents for 10 plates.

50mM Carbonate/Bicarbonate buffer, pH 9.6

Phosphate Buffered Saline, pH 7.4, containing 0.05% Tween 20 (PBS-T)

Bovine Serum Albumin

Streptavidin-Peroxidase

1mM ABTS in 70mM citrate-phosphate buffer, pH 4.2

EL-BV1 Certificate of Analysis

Bet v 1 Standard Letter - October 2017

1. Breiteneder H, Pettenburger K, Bito A, et al. The gene coding for themajor birch pollen allergen Bet v 1, is highly homologous to a peadisease resistance response gene. EMBO J. 1989;8:1935-8. 

2. Hoffmann-Sommergruber K, Susani M, Ferreira F, et al. High-levelexpression and purification of the major birch pollen allergen, Bet v 1.Protein Expr Purif. 1997;9:33-9. 

3. Godnic-Cvar J, Susani M, Breiteneder H, et al. Recombinant Bet v 1, themajor birch pollen allergen, induces hypersensitivity reactions equal tothose induced by natural Bet v 1 in the airways of patients allergic to treepollen. J Allergy Clin Immu nol. 1997;99:354-9. 

4. Schappi GF, Monn C, Wuthrich B, Wanner HU. Direct determination ofallergens in ambient aerosols: methodological aspects. Int Arch AllergyImmunol. 1996;110:364-70. 

5. Schappi GF, Suphioglu C, Taylor PE, Knox RB. Concentrations of themajor birch tree allergen Bet v 1 in pollen and respirable fine particles inthe atmosphere. J Allergy Clin Immunol. 1997;100:656-61. 

6. S. Vieths, D. Barber, M. Chapman, et al. Establishment of recombinantmajor allergens Bet v 1 and Phl p 5a as Ph. Eur. Reference standardsand validation of ELISA methods for their measurement. Pharmeuropa Bio&SN October 2012;118-13.