Monoclonal antibody 1D11, Phl p 5 Standard, Biotinylated antibody Bo1; sufficient reagents for 10 plates.

50mM Carbonate/Bicarbonate buffer, pH 9.6

Phosphate Buffered Saline, pH 7.4, containing 0.05% Tween 20 (PBS-T)

Bovine Serum Albumin

Streptavidin-Peroxidase

1mM ABTS in 70mM citrate-phosphate buffer, pH 4.2

For research and commercial use in vitro: not for human in vivo or therapeutic use.

Binds to species specific epitope present on timothy grass pollen allergen, Phl p 5a & b.

Binds to species specific epitope present on timothy grass pollen allergen, Phl p 5a & b.

References:

1. Müller WD, Diener C, Jung K, Jäger L. Antigens of Timothy and othergrass pollen extracts identified by monoclonal antibodies. AllergolImmunopathol (Madr). 1988;16:315-20. 

2. Fahlbusch B, Müller WD, Diener CH, Jäger L. Detection of crossreactivedeterminants in grass pollen extracts using monoclonal antibodiesagainst group IV and group V allergens. Clin Exp Allergy. 1993;23:51-60. 

3. Petersen A, Becker WM, Schlaak M. Epitope analysis of isoforms of themajor allergen Phl p V by fingerprinting and microsequencing. Clin ExpAllergy. 1994;24:250-6. 

4. S. Vieths, D. Barber, M. Chapman, A. Costanzo, A. Daas, H. Fiebig, K.M.Hanschmann, M. Hrabina, S. Kaul, A. Ledesma, P. Moingeon, G. Reese,C. Schörner, R. van Ree, B. Weber, K.H. Buchheit. Establishment ofrecombinant major allergens Bet v 1 and Phl p 5a as Ph. Eur. Referencestandards and validation of ELISA methods for their measurement.Pharmeuropa Bio&SN October 2012; 118-13.