Purification:

Multi-step chromatographic purification. Purity > 95 % by SDS-PAGE

See product insert.

 In sterile, endotoxin free 1x PBS, pH 7.4

(1) A LoTox™ product: Endotoxin <0.03 EU/µg.

(2) LoTox^TM rPhl p 5a has a C-terminal 7xHis-tag

1. Niederberger V, Laffer S, Froschl R, Kraft D, et al. IgE antibodies to recombinant pollen allergens (Phl p 1, Phl p 2, Phl p 5, and Bet v 2) account for a high percentage of grass pollen-specific IgE. J Allergy Clin Immunol 1998;101:258-64.

2. Mari A. Skin test with a timothy grass (Phleum pratense) pollen extract vs. IgE to a timothy extract vs. IgE to rPhl p 1, rPhl p 2, nPhl p 4, rPhl p 5, rPhl p 6, rPhl p 7, rPhl p 11, and rPhl p 12: epidemiological and diagnostic data. Clin Exp Allergy 2003;33:43-51.

3. Jutel M, Jaeger L, Suck R, et al. Allergen-specific immunotherapy with recombinant grass pollen allergens. J Allergy Clin Immunol 2005;116:608-13.