Multi-step chromatographic purification. Purity > 95 % by SDS-PAGE
See product insert.
Reconstitute using sterile, endotoxin free PBS, pH 7.4, at a concentration up to 1mg/ml. 241 amino acids, Molar ext. coeff: 45340; 1.0 A280 = 0.57mg/ml
Freeze dried in glass ampule
Store at -20ºC, before and after reconstitution
A LoTox™ product: Endotoxin <0.03 EU/µg
Peterson A, Schramm G, Bufe A, Schlaak M, Becker W. Structural investigations of the major allergen Phl p 1 on the complementary DNA and protein level. J Allergy Clin Immunol 1995;95:987-94
Mari A. Skin test with a timothy grass (Phleum pratense) pollen extract vs. IgE to a timothy extract vs. IgE to rPhl p 1, rPhl p 2, nPhl p 4, rPhl p 5, rPhl p 6, rPhl p 7, rPhl p 11, and rPhl p 12: epidemiological and diagnostic data. Clin Exp Allergy 2003;33:43-51. Jutel M, Jaeger L, Suck R, et al. Allergen-specific immunotherapy with recombinant grass pollen allergens. J Allergy Clin Immunol 2005;116:608-13.