ELISA kit for measuring S. chartarum antigen.
Limit of Detection:
31U/ml
Assay Performance:
Inquire
Contents:
Monoclonal antibody 3F10, Stachybotrys chartarum Standard, Biotinylated antibody 3F10; sufficient reagents for 10 microtiter plates.
Not Provided:
50mM carbonate/bicarbonate buffer, pH9.6
Phosphate buffered saline, pH 7.4, containing 0.05% Tween 20 (PBS-T)
Bovine serum albumin
Streptavidin-Peroxidase
1mM ABTS in 70mM citrate-phosphate buffer, pH 4.2
For research and commercial use in vitro: not for human in vivo or therapeutic use.
Monoclonal Antibody:
3F10 (clone 3F10 A5 B10)
Immunogen:
Stachybotrys chartarum extract
Specificity:
Binds to a species specific epitope of Stachybotrys chartarum antigen.
Purification:
Produced in tissue culture and purified by chromatography using Protein A.
Single heavy and light chain bands on SDS-PAGE.
Concentration:
1mg/ml in phosphate buffered saline, pH 7.4. Based on A280 for IgG (1.42=1mg/ml), 0.22μm filtered, preservative free.
Monoclonal Antibody:
3F10 (clone 3F10 A5 B10)
Immunogen:
Stachybotrys chartarum extract
Specificity:
Binds to a species specific epitope of Stachybotrys chartarum antigen.
Purification:
Produced in tissue culture and purified by chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE.
Biotinylation:
Biotinylated and titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22μm filtered, preservative free.
Antigen Standard:
Stachybotrys chartarum
Composition:
Stachybotrys chartarum culture extract prepared in 1% BSA/50% glycerol/PBS, 0.22μm filtered, preservative free, pH 7.4.
Concentration:
10,000U/ml SchY
Calibration:
The concentration of the extract was measured using a total protein assay. The extract is calibrated in arbitrary units where 1 unit of SchY equals approximately 1 nanogram of total protein.