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    产品货号:EL-BD2
    促销: 12720.00
    价格: 12720.00

    分享

    kit
    美国Indoor

    ELISA kit for measuring bovine Bos domesticus dander allergen, Bos d 2.

    Antibodies:
    3D4/mAb1

    Sensitivity:
    0.1 ng/ml

    Limit of Detection:

    0.4 ng/ml

    Contents:

    Monoclonal antibody 3D4, Bos d 2 Standard, Biotinylated antibody mAb1; sufficient reagents for 10 microtiter plates.

    Storage:
    Store at 4°C
    Not Provided:

    50mM carbonate/bicarbonate buffer, pH 9.6
    Phosphate buffered saline, pH 7.4, containing 0.05% Tween 20 (PBS-T)
    Bovine serum albumin
    Streptavidin-Peroxidase
    1mM ABTS in 70mM citrate-phosphate buffer, pH 4.2

    Notes:

    The Bovine Allergen Standard is recommended for immunoassay calibration purposes only. Not recommended for in-vitro antibody measurements, T cell studies, immunization purposes, or other uses.

    For research and commercial use in vitro: not for human in vivo or therapeutic
    use.

    Certificate of Analysis

    Monoclonal Antibody:
    3D4

    Vial:
    Red Cap; 100µl

    Immunogen:
    Bovine dander allergen

    Isotype:
    IgG1

    Specificity:

    Binds to an epitope on bovine Bos domesticus dander allergen, Bos d 2.

    Purification:

    Produced in tissue culture and purified by affinity chromatography using recombinant Protein G. Single heavy and light chain bands on SDS-PAGE.

    Concentration:

    1.33 mg/ml in phosphate buffered saline, pH 7.4. Based on A280 for IgG (1.42=1mg/ml), 0.22μm filtered, preservative free.



    Certificate of Analysis

    Monoclonal Antibody:
    mAb1

    Vial:
    Brown Cap; 100µl

    Immunogen:
    Bovine dander allergen

    Isotype:
    IgG2b

    Specificity:

    Binds to an epitope on bovine Bos domesticus dander

    allergen, Bos d 2.

    Purification:

    Produced in tissue culture and purified by affinity chromatography using recombinant Protein G. Single heavy and light chain bands on SDS-PAGE.

    Biotinylation:

    Biotinylated and titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22μm filtered, preservative free.



    Certificate of Analysis

    Allergen Standard:
    Bos d 2 Standard

    Vial:
    White Cap; 400µl

    Composition:

    Recombinant Bos d 2 allergen was prepared in 0.5% HSA/50% glycerol/PBS, pH 7.4.

    Concentration/Calibration: 
    500 ng/ml. The concentration of the purified recombinant Bos d 2 was determined by amino acid analysis. There are no national or international Reference standards for Bos d 2.

    References:

    1.  Ylönen J, Virtanen T, Rytkönen M, Mäntyjärvi R. Affinity purification of the major bovine allergen by a novel monoclonal antibody. J Allergy Clin Immunol. 1994 May;93(5):851-8.


    2.  Ylönen J, Virtanen T, Rytkönen M, Mäntyjärvi R. Quantification of a major bovine allergen by a two-site immunometric assay based on monoclonal antibodies. Allergy. 1994 Oct;49(9):707-12.


    3.  Rouvinen J, Rautiainen J, Virtanen T, Zeiler T, Kauppinen J, Taivainen A, Mäntyjärvi R. Probing the molecular basis of Allergy: Three-dimensional structure of the bovine lipocalin allergen Bos d 2. The Journal of Biological Chemistry 1999;274(4):2337-43.


    4.  Saarelainen SA, Kinnunen TT, Buhot C, Närvänen AT, Kauppinen AK, Rytkönen-Nissinen MA, Maillere B, Virtanen TI. Immunotherapeutic potential of the immunodominant T-cell epitope of lipocalin allergen Bos d 2 and its analogues. Immunology. 2008 Mar;123(3):358-66.


    5.  Filep S, Tsay A, Vailes LD, Gadermaier G, Ferreira F, Matsui EC et al. A multi-allergen standard for immunoassays: CREATE principles applied to eight purified allergens. Allergy. 18 Nov 2011; DOI: 10.1111/j. 1398-9995.2011.02750x.


    6.  Kauppinen A, Peräsaari J, Taivainen A, Kinnunen T, Saarelainen S, Rytkönen-Nissinen M, Jeal H, Jones M, Virtanen T. Association of HLA class II alleles with sensitization to cow dander Bos d 2, an important occupational allergen. Immunobiology. 2012 Jan; 217(1):8-12.

    (For complete reference list see EL-BD2 Certificate of Analysis)